UCLA researchers have discovered that a combination of drugs, electrical stimulation and regular exercise can enable paralyzed rats to walk and even run while supporting their full weight on a treadmill.
Published Sept. 20 in the online edition of the journal Nature Neuroscience, the findings suggest that the regeneration of severed nerve fibers is not required for paraplegic rats to learn to walk again. The research may hold implications for rehabilitation after human spinal cord injuries.
"The spinal cord contains nerve circuits that can generate rhythmic activity without input from the brain to drive the hind leg muscles in a way that resembles walking, called ’stepping,’ " said principal investigator Reggie Edgerton, a professor of neurobiology at the David Geffen School of Medicine at UCLA and professor of physiological sciences at the UCLA College of Letters and Science.
"Previous studies have tried to tap into this circuitry to help victims of spinal cord injury," he added. "While other researchers have elicited similar leg movements in people with complete spinal injuries, they have not achieved full weight–bearing and sustained stepping as we have in our study."
Edgerton’s team tested rats with complete spinal injuries that left no voluntary movement in their hind legs. After setting the paralyzed rats on a moving treadmill belt, the scientists administered drugs that act on the neurotransmitter serotonin and applied low levels of electrical currents to the spinal cord below the point of injury.
The combination of stimulation and sensation derived from the rats’ limbs moving on a treadmill belt triggered the spinal rhythm–generating circuitry and prompted walking motion in the rats’ paralyzed hind legs.
Daily treadmill training over several weeks eventually enabled the rats to regain full weight–bearing walking, including backwards, sideways and at running speed. However, the injury still interrupted the brain’s connection to the spinal cord–based rhythmic walking circuitry, leaving the rats unable to walk of their own accord.
In humans, however, neuroprosthetic devices may bridge spinal cord injuries to some extent, so activating the spinal cord rhythmic circuitry as the UCLA team did may help in rehabilitation after spinal cord injuries.
The study was funded by the Christopher and Dana Reeve Foundation, the Craig Nielsen Foundation, the National Institute of Neurological Disorders and Stroke, the U.S. Civilian Research and Development Foundation, the International Paraplegic Foundation, the Swiss National Science Foundation, the National Institute of Biomedical Imaging and Bioengineering, the Roman Reed Spinal Cord Injury Research Fund of California and the Russian Foundation for Basic Research Grants.