Previous work by the group of Professor Dr Cyril Zipfel at The Sainsbury Laboratory (Norwich, UK) and now at the University of Zürich (Zürich, Switzerland) had identified that RALF23 regulates plant innate immunity. Using a combination of genetics, biochemistry and structural biology, a close collaboration between this group and the group of Professor Dr Jijie Chai at the Innovation Center for Structural Biology and the Joint Center for Life Sciences of Tsinghua and Peking Universities (Beijing, China) and at the University of Cologne (Cologne, Germany) has now identified the molecular basis for RALF23 perception. This work further involved collaborators from the Gregor Mendel Institute (Vienna, Austria).
Professor Jijie Chai said: ‘We were excited about the results, when we saw that RALF23 needs two distinct types of proteins - a receptor kinase (FERONIA) and an unrelated membrane-associated protein - to be recognized. The way these three proteins form an impressive perception complex might apply to other plant receptors that recognize peptide hormones.’
Professor Cyril Zipfel added: ‘FERONIA is a plant receptor that was actually identified at the University of Zürich over a decade ago by my colleague Professor Grossniklaus for its important role in reproduction, but has since been shown to play key roles in multiple plant processes. Now that we understand the molecular basis of how FERONIA can perceive RALF peptides, it will help characterize how this unique receptor controls several aspects of plants’ life.’
Jijie Chai is Alexander von Humboldt Professor at the University of Cologne. At the University and at the Max Planck Institute for Plant Breeding Research in Cologne, he conducts research on the structure of proteins and receptors that are important for the immune defence of living organisms. Whether in humans, mice or grains, proteins are very similar across animal and plant cells. Understanding these proteins is a key to influencing their immune defence. By exploring the complex structures of proteins, Chai provides important fundamental research for the fight against plant diseases and the development of new drugs, for example in the treatment of inflammatory diseases.