Amphipods are collected from groundwater and then genetically characterised.
Switzerland’s groundwater is home to a multitude of hitherto unknown organisms. An Eawag research project is shining a light into the darkness and revealing this habitat’s exceptional biodiversity.
Switzerland has plentiful groundwater reserves. Found in cavities under the earth, groundwater is almost ubiquitously present, and is the country’s biggest source of drinking water. Unlike the drinking water that comes out of our taps, however, groundwater is also a natural habitat and, as such, is home to a wide range of organisms - from the smallest of microorganisms to larger creatures such as amphipods.
Amphipods are abundant in groundwater. Since they live in a dark environment, they lack pigments.
"Generally speaking, the fact that there are creatures living in the groundwater is a good sign," explains Florian Altermatt, "as these organisms are dependent on a high level of water quality. However, it is not entirely clear at what levels of pollution they start to be affected." Altermatt is a Research Group Leader at the aquatic research institute Eawag and a professor at the University of Zurich. As yet, scientists know very little about the identity and distribution of groundwater organisms, so the team have set themselves the task of researching the groundwater ecosystem in greater detail. Altermatt’s team is therefore conducting the first Switzerland-wide study of biodiversity in groundwater - the "AmphiWell" project - as part of the Naqua National Groundwater Monitoring programme on behalf of the Federal Office for the Environment (FOEN).
Strong support from the water supply companies
Mara Knüsel, who is working on the project for her PhD thesis, explains the project’s approach: "Although groundwater is present everywhere beneath our feet, it’s very hard for us to get at. The best way to access it is via Switzerland’s countless spring boxes." However, these spring boxes are not accessible to the general public and are maintained by the water supply companies. The researchers are therefore working with the water industry (water supply managers, water control officers, private spring operators, etc.) to collect as many samples as possible from different locations as part of a citizen science project. Of the 700 water utilities approached, almost half have already agreed to take part. "They’re very keen and happy to be involved, and they’re also interested in the results," explains Knüsel. "We’re really lucky to be able to sample the spring boxes in this way."
Using filter nets attached directly to the inlet points, a number of organisms - above all amphipods - are collected over the course of a week. The animals are then preserved in ethanol and sent to Eawag. Genetic analysis allows the collected amphipods to be assigned to a known - or new - species.
In the project video (see below), we see a water supply manager fitting a filter net directly to an inlet point. The net collects a number of organisms - above all amphipods - over the course of a week, which are then preserved in ethanol in a sample tube and sent to Eawag. Genetic analysis allows the collected amphipods to be assigned to a known - or new - species, and Knüsel then notifies the water utilities of the organisms that were found in the net.
Studer, A.; Knüsel, M.; Alther, R.; Hürlemann, S.; Altermatt, F. (2022) Erfassung der Grundwasserflohkrebse. Studie zur Artenvielfalt und Verbreitung im Einzugsgebiet der Töss, Aqua & Gas
, 102(4), 14-19 , Institutional Repository