PicoRuler: Protein-based molecular rulers make it possible to test the optical resolution of the latest super-resolution microscopy methods on biomolecules in the sub-10-nanometre range under realistic conditions. (Image: Gerti Beliu / Universität Würzburg, erstellt mit DALL·E 3)
PicoRuler: Protein-based molecular rulers make it possible to test the optical resolution of the latest super-resolution microscopy methods on biomolecules in the sub-10-nanometre range under realistic conditions. (Image: Gerti Beliu / Universität Würzburg, erstellt mit DALL·E 3) - Good news for researchers working with high-resolution fluorescence microscopy: Biocompatible molecular rulers are available for the first time to calibrate the latest super-resolution microscopy methods. Latest super-resolution microscopy methods now achieve an optical resolution in the range of a few nanometres. This corresponds to a resolution in the range of the size of cellular molecules. However, it has not yet been possible to verify the resolution actually achieved on cellular building blocks such as multiprotein complexes - because there were no biomolecular reference systems that could be labelled with dyes at precisely defined positions at a distance of a few nanometres. A team led by Dr Gerti Beliu and Professor Markus Sauer from the Rudolf Virchow Centre - Center for Integrative and Translational Bioimaging at Julius-Maximilians-Universität (JMU) Würzburg in Bavaria, Germany, has now provided a turning point. In the journal Advanced Materials , they present novel biocompatible molecular rulers, the PicoRulers (Protein-based Imaging Calibration Optical Rulers).
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