Parallel reporter assay developed by Hartl and colleagues.
Transcription of our genes mostly begins in regions of the genome called CpG islands. These are rich in the dinucleotide CpG (thus the name), critical for gene activity and devoid of DNA methylation. Despite the relevance of CpG islands, it is unclear if the CpG dinucleotide itself contributes to their activity. This is what researchers from the Schübeler group investigated. Their findings refine our understanding of mammalian promoter regulation. Gene expression is controlled at multiple levels, including recognition of specific DNA sequences by transcription factors, packaging of DNA in chromatin and methylation of DNA. While the majority of transcription factors recognize complex motifs of several nucleotides, it is largely open whether low complexity sequence features, such as dinucleotides, contribute independently to gene activity.
TO READ THIS ARTICLE, CREATE YOUR ACCOUNT
And extend your reading, free of charge and with no commitment.
