FMI mouse intestinal organoids made it to the Nature cover!
FMI mouse intestinal organoids made it to the Nature cover! - Intestinal organoids recapitulate not only the structure of intestinal epithelium but also its ability to regenerate following damage. Using this research tool, the group of Prisca Liberali unraveled mechanisms orchestrating organoid formation and intestinal regeneration with a unique image-based screening approach. The researchers identified a compound that improves the regeneration of the intestine in vivo . The last decade has seen a boom in the field of organoids, miniature organs grown from stem cells in vitro. These systems recapitulate the cell type composition and numerous functions of parent organs - such as brain, kidney, intestine or lung - and are perfectly suited for experimental manipulations, making them invaluable tools for researchers worldwide. Organoids from the intestine - the fastest renewing tissue in mammals - recapitulate not only the structure of intestinal epithelium but also its ability to regenerate following damage. Intestinal organoids can develop from a single cell, driven by the intrinsic capacity of a cell to undergo a regenerative process, building a complicated hierarchical structure through self-organization. However, the factors that drive and regulate this process are not well understood. Researchers from the group of Prisca Liberali set out to understand intestinal regeneration by mapping the functional genetic interactions regulating this process. For this, they established an image-based phenotypic screening platform, profiling over 400,000 organoids treated with a library of compounds to assess which compounds affect the organoids. They then classified every organoid by phenotype, generating a unique "phenotypic fingerprint?
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